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Product Info Summary
| SKU: | A11558-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ABRA Antibody Picoband™
SKU/Catalog Number
A11558-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ABRA Antibody Picoband™ catalog # A11558-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ABRA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11558-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ABRA recombinant protein (Position: A13-K381).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A11558-1 is reactive to ABRA in Human, Mouse, Rat
Applications
A11558-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
43.117kDa
Background of ABRA
Predicted to enable actin binding activity. Predicted to be involved in positive regulation of DNA-binding transcription factor activity; positive regulation of Rho protein signal transduction; and positive regulation of transcription by RNA polymerase II. Predicted to act upstream of or within positive regulation of transcription, DNA-templated and protein import into nucleus. Located in plasma membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ABRA using anti-ABRA antibody (A11558-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart tissue lysates,
Lane 3: mouse skeletal muscle tissue lysates,
Lane 4: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABRA antigen affinity purified polyclonal antibody (Catalog # A11558-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABRA at approximately 39 kDa. The expected band size for ABRA is at 39 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ABRA (Source: Uniprot.org, NCBI)
Gene Name
ABRA
Full Name
Actin-binding Rho-activating protein
Weight
43.117kDa
Alternative Names
actin-binding Rho activating protein; actin-binding Rho-activating protein; STARSStriated muscle activator of Rho-dependent signaling ABRA STARS actin binding Rho activating protein actin-binding Rho-activating protein|striated muscle activator of Rho-dependent signaling
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABRA, check out the ABRA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABRA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABRA Antibody Picoband™ (A11558-1)
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No publications found for A11558-1
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Customer Reviews
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